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sds page gel preparation kit  (Beyotime)


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    Structured Review

    Beyotime sds page gel preparation kit
    Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). <t>(F)</t> <t>SDS-PAGE</t> analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.
    Sds Page Gel Preparation Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+page+gel+preparation+kit/TBE+PAGE+Gel+Preparation+Kit/pmc12856548-373-25-32
    Average 99 stars, based on 382 article reviews
    sds page gel preparation kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis"

    Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.11.047

    Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). (F) SDS-PAGE analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.
    Figure Legend Snippet: Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). (F) SDS-PAGE analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.

    Techniques Used: Zeta Potential Analyzer, Fluorescence, Microscopy, SDS Page, Membrane, Staining, Labeling, Conjugation Assay, In Vitro

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    Lysis:

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    SDS Page:

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    Article Title: Identifying a Marine-Derived Small-Molecule Nucleoprotein Inhibitor Against Influenza A Virus
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    Activity Assay:

    Article Title: Piperlongumine Inhibits OSCC Epithelial-Mesenchymal Transition and Promotes Tumor Cell Apoptosis by Targeting TrxR1 to Activate ROS and Its Related Pathways
    Article Snippet: Piperlongumine (PL), a naturally occurring alkaloid isolated from Piper longum fruit, has been shown to selectively toxinate a variety of tumor cells.. However, PL has received less attention when it comes to oral squamous cell carcinoma (OSCC), and its precise effects and mechanisms have not been completely clarified.. We verified that thioredoxin reductase 1 (TrxR1) is highly expressed in OSCC cell lines and cancer tissues of OSCC patients; PL inhibited TrxR1 activity in a concentration-dependent manner; PL inhibited OSCC cells in a concentration-dependent manner, and the inhibitory effect was more pronounced in TrxR1 low-regulated expression cells; PL could cause an increase in the level of reactive oxygen specie (ROS) in OSCC cells, and the effect was more pronounced in TrxR1 low-expression OSCC cells; PL inhibited the activation of the WNT pathway to prevent the occurrence of the epithelialmesenchymal transition (EMT) in OSCC cells; PL activated the activation of the mitochondrial apoptotic pathway to promote the occurrence of OSCC apoptosis; and both the inhibition of the WNT pathway and the activation of the mitochondrial apoptosis by PL could be reversed by the ROS scavenger GSH.

    ROS Assay:

    Article Title: Piperlongumine Inhibits OSCC Epithelial-Mesenchymal Transition and Promotes Tumor Cell Apoptosis by Targeting TrxR1 to Activate ROS and Its Related Pathways
    Article Snippet: Piperlongumine (PL), a naturally occurring alkaloid isolated from Piper longum fruit, has been shown to selectively toxinate a variety of tumor cells.. However, PL has received less attention when it comes to oral squamous cell carcinoma (OSCC), and its precise effects and mechanisms have not been completely clarified.. We verified that thioredoxin reductase 1 (TrxR1) is highly expressed in OSCC cell lines and cancer tissues of OSCC patients; PL inhibited TrxR1 activity in a concentration-dependent manner; PL inhibited OSCC cells in a concentration-dependent manner, and the inhibitory effect was more pronounced in TrxR1 low-regulated expression cells; PL could cause an increase in the level of reactive oxygen specie (ROS) in OSCC cells, and the effect was more pronounced in TrxR1 low-expression OSCC cells; PL inhibited the activation of the WNT pathway to prevent the occurrence of the epithelialmesenchymal transition (EMT) in OSCC cells; PL activated the activation of the mitochondrial apoptotic pathway to promote the occurrence of OSCC apoptosis; and both the inhibition of the WNT pathway and the activation of the mitochondrial apoptosis by PL could be reversed by the ROS scavenger GSH.

    Apoptosis Assay:

    Article Title: Piperlongumine Inhibits OSCC Epithelial-Mesenchymal Transition and Promotes Tumor Cell Apoptosis by Targeting TrxR1 to Activate ROS and Its Related Pathways
    Article Snippet: Piperlongumine (PL), a naturally occurring alkaloid isolated from Piper longum fruit, has been shown to selectively toxinate a variety of tumor cells.. However, PL has received less attention when it comes to oral squamous cell carcinoma (OSCC), and its precise effects and mechanisms have not been completely clarified.. We verified that thioredoxin reductase 1 (TrxR1) is highly expressed in OSCC cell lines and cancer tissues of OSCC patients; PL inhibited TrxR1 activity in a concentration-dependent manner; PL inhibited OSCC cells in a concentration-dependent manner, and the inhibitory effect was more pronounced in TrxR1 low-regulated expression cells; PL could cause an increase in the level of reactive oxygen specie (ROS) in OSCC cells, and the effect was more pronounced in TrxR1 low-expression OSCC cells; PL inhibited the activation of the WNT pathway to prevent the occurrence of the epithelialmesenchymal transition (EMT) in OSCC cells; PL activated the activation of the mitochondrial apoptotic pathway to promote the occurrence of OSCC apoptosis; and both the inhibition of the WNT pathway and the activation of the mitochondrial apoptosis by PL could be reversed by the ROS scavenger GSH.

    Membrane:

    Article Title: Piperlongumine Inhibits OSCC Epithelial-Mesenchymal Transition and Promotes Tumor Cell Apoptosis by Targeting TrxR1 to Activate ROS and Its Related Pathways
    Article Snippet: Piperlongumine (PL), a naturally occurring alkaloid isolated from Piper longum fruit, has been shown to selectively toxinate a variety of tumor cells.. However, PL has received less attention when it comes to oral squamous cell carcinoma (OSCC), and its precise effects and mechanisms have not been completely clarified.. We verified that thioredoxin reductase 1 (TrxR1) is highly expressed in OSCC cell lines and cancer tissues of OSCC patients; PL inhibited TrxR1 activity in a concentration-dependent manner; PL inhibited OSCC cells in a concentration-dependent manner, and the inhibitory effect was more pronounced in TrxR1 low-regulated expression cells; PL could cause an increase in the level of reactive oxygen specie (ROS) in OSCC cells, and the effect was more pronounced in TrxR1 low-expression OSCC cells; PL inhibited the activation of the WNT pathway to prevent the occurrence of the epithelialmesenchymal transition (EMT) in OSCC cells; PL activated the activation of the mitochondrial apoptotic pathway to promote the occurrence of OSCC apoptosis; and both the inhibition of the WNT pathway and the activation of the mitochondrial apoptosis by PL could be reversed by the ROS scavenger GSH.

    Article Title: Electroacupuncture pretreatment alleviates cerebral Ischemia-Reperfusion-Induced intestinal barrier injury and neuroinflammation via inhibition of the TLR4/NLRP3 inflammasome pathway in rats.
    Article Snippet: This study investigates the protective effects and mechanisms of electroacupuncture (EA) pretreatment on intestinal barrier dysfunction following cerebral ischemia–reperfusion injury (CIRI), with a focus on the TLR4/ NLRP3 inflammasome pathway.. A middle cerebral artery occlusion (MCAO) rat model was established, with groups divided into Sham, ischemia–reperfusion (I/R), and EA pretreatment (I/R + EA).. Neurological function and intestinal pathology were dynamically assessed at 2, 24, and 72 h post-reperfusion.

    Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis
    Article Snippet: Bortezomib (BTZ) and Sabutoclax (Sab) were purchased from Selleck Biotech (USA). .. Hoechst 33342, DAPI solution, Lyso-Tracker Green, Cell Counting Kit-8 (CCK-8), Calcein-AM/PI Live/Dead cell double staining kit, membrane and cytosol protein extraction kit, BCA kit, and SDS-PAGE gel preparation kit were procured from Beyotime Biotechnology Co., Ltd. (Shanghai, China). .. Dimethylmethylene blue (DMMB) assay kit was procured from HePengBiotech, Ltd (Shanghai, China).

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    Article Snippet: Hydrogen tetrachloroaurate(III) trihydrate (HAuCl4·3H2O) and triphenylphosphine were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). .. The mitochondrial green fluorescent probe JC-1, Enhanced Mitochondrial Membrane Potential Assay Kit (JC-1), SDS-PAGE Gel Preparation Kit, BeyoColor Prestained Protein Marker, RIPA Lysis Buffer (Strong), PMSF, BCA Protein Concentration Assay Kit (Enhanced), HRP-conjugated Goat Anti-Mouse IgG (H + L), HRP-conjugated Goat Anti-Rabbit IgG (H + L), QuickBlock Western Primary Antibody Dilution Buffer, QuickBlock Western Secondary Antibody Dilution Buffer, Western Wash Buffer (10X), QuickBlock Western Blocking Buffer, and Ultrasensitive ECL Chemiluminescence Kit were acquired from Beyotime Biotechnology Co., Ltd. (Shanghai, China). ..

    Staining:

    Article Title: Piperlongumine Inhibits OSCC Epithelial-Mesenchymal Transition and Promotes Tumor Cell Apoptosis by Targeting TrxR1 to Activate ROS and Its Related Pathways
    Article Snippet: Piperlongumine (PL), a naturally occurring alkaloid isolated from Piper longum fruit, has been shown to selectively toxinate a variety of tumor cells.. However, PL has received less attention when it comes to oral squamous cell carcinoma (OSCC), and its precise effects and mechanisms have not been completely clarified.. We verified that thioredoxin reductase 1 (TrxR1) is highly expressed in OSCC cell lines and cancer tissues of OSCC patients; PL inhibited TrxR1 activity in a concentration-dependent manner; PL inhibited OSCC cells in a concentration-dependent manner, and the inhibitory effect was more pronounced in TrxR1 low-regulated expression cells; PL could cause an increase in the level of reactive oxygen specie (ROS) in OSCC cells, and the effect was more pronounced in TrxR1 low-expression OSCC cells; PL inhibited the activation of the WNT pathway to prevent the occurrence of the epithelialmesenchymal transition (EMT) in OSCC cells; PL activated the activation of the mitochondrial apoptotic pathway to promote the occurrence of OSCC apoptosis; and both the inhibition of the WNT pathway and the activation of the mitochondrial apoptosis by PL could be reversed by the ROS scavenger GSH.

    Protein Extraction:

    Article Title: Electroacupuncture pretreatment alleviates cerebral Ischemia-Reperfusion-Induced intestinal barrier injury and neuroinflammation via inhibition of the TLR4/NLRP3 inflammasome pathway in rats.
    Article Snippet: This study investigates the protective effects and mechanisms of electroacupuncture (EA) pretreatment on intestinal barrier dysfunction following cerebral ischemia–reperfusion injury (CIRI), with a focus on the TLR4/ NLRP3 inflammasome pathway.. A middle cerebral artery occlusion (MCAO) rat model was established, with groups divided into Sham, ischemia–reperfusion (I/R), and EA pretreatment (I/R + EA).. Neurological function and intestinal pathology were dynamically assessed at 2, 24, and 72 h post-reperfusion.

    Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis
    Article Snippet: Bortezomib (BTZ) and Sabutoclax (Sab) were purchased from Selleck Biotech (USA). .. Hoechst 33342, DAPI solution, Lyso-Tracker Green, Cell Counting Kit-8 (CCK-8), Calcein-AM/PI Live/Dead cell double staining kit, membrane and cytosol protein extraction kit, BCA kit, and SDS-PAGE gel preparation kit were procured from Beyotime Biotechnology Co., Ltd. (Shanghai, China). .. Dimethylmethylene blue (DMMB) assay kit was procured from HePengBiotech, Ltd (Shanghai, China).

    Marker:

    Article Title: Electroacupuncture pretreatment alleviates cerebral Ischemia-Reperfusion-Induced intestinal barrier injury and neuroinflammation via inhibition of the TLR4/NLRP3 inflammasome pathway in rats.
    Article Snippet: This study investigates the protective effects and mechanisms of electroacupuncture (EA) pretreatment on intestinal barrier dysfunction following cerebral ischemia–reperfusion injury (CIRI), with a focus on the TLR4/ NLRP3 inflammasome pathway.. A middle cerebral artery occlusion (MCAO) rat model was established, with groups divided into Sham, ischemia–reperfusion (I/R), and EA pretreatment (I/R + EA).. Neurological function and intestinal pathology were dynamically assessed at 2, 24, and 72 h post-reperfusion.

    Article Title: Mitochondria-Targeted Au@Carbon Dot Nanoprobes for SERS Analysis of Drug-Induced Mitophagy
    Article Snippet: Hydrogen tetrachloroaurate(III) trihydrate (HAuCl4·3H2O) and triphenylphosphine were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). .. The mitochondrial green fluorescent probe JC-1, Enhanced Mitochondrial Membrane Potential Assay Kit (JC-1), SDS-PAGE Gel Preparation Kit, BeyoColor Prestained Protein Marker, RIPA Lysis Buffer (Strong), PMSF, BCA Protein Concentration Assay Kit (Enhanced), HRP-conjugated Goat Anti-Mouse IgG (H + L), HRP-conjugated Goat Anti-Rabbit IgG (H + L), QuickBlock Western Primary Antibody Dilution Buffer, QuickBlock Western Secondary Antibody Dilution Buffer, Western Wash Buffer (10X), QuickBlock Western Blocking Buffer, and Ultrasensitive ECL Chemiluminescence Kit were acquired from Beyotime Biotechnology Co., Ltd. (Shanghai, China). ..

    CCK-8 Assay:

    Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis
    Article Snippet: Bortezomib (BTZ) and Sabutoclax (Sab) were purchased from Selleck Biotech (USA). .. Hoechst 33342, DAPI solution, Lyso-Tracker Green, Cell Counting Kit-8 (CCK-8), Calcein-AM/PI Live/Dead cell double staining kit, membrane and cytosol protein extraction kit, BCA kit, and SDS-PAGE gel preparation kit were procured from Beyotime Biotechnology Co., Ltd. (Shanghai, China). .. Dimethylmethylene blue (DMMB) assay kit was procured from HePengBiotech, Ltd (Shanghai, China).

    Double Staining:

    Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis
    Article Snippet: Bortezomib (BTZ) and Sabutoclax (Sab) were purchased from Selleck Biotech (USA). .. Hoechst 33342, DAPI solution, Lyso-Tracker Green, Cell Counting Kit-8 (CCK-8), Calcein-AM/PI Live/Dead cell double staining kit, membrane and cytosol protein extraction kit, BCA kit, and SDS-PAGE gel preparation kit were procured from Beyotime Biotechnology Co., Ltd. (Shanghai, China). .. Dimethylmethylene blue (DMMB) assay kit was procured from HePengBiotech, Ltd (Shanghai, China).

    Protein Concentration:

    Article Title: Mitochondria-Targeted Au@Carbon Dot Nanoprobes for SERS Analysis of Drug-Induced Mitophagy
    Article Snippet: Hydrogen tetrachloroaurate(III) trihydrate (HAuCl4·3H2O) and triphenylphosphine were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). .. The mitochondrial green fluorescent probe JC-1, Enhanced Mitochondrial Membrane Potential Assay Kit (JC-1), SDS-PAGE Gel Preparation Kit, BeyoColor Prestained Protein Marker, RIPA Lysis Buffer (Strong), PMSF, BCA Protein Concentration Assay Kit (Enhanced), HRP-conjugated Goat Anti-Mouse IgG (H + L), HRP-conjugated Goat Anti-Rabbit IgG (H + L), QuickBlock Western Primary Antibody Dilution Buffer, QuickBlock Western Secondary Antibody Dilution Buffer, Western Wash Buffer (10X), QuickBlock Western Blocking Buffer, and Ultrasensitive ECL Chemiluminescence Kit were acquired from Beyotime Biotechnology Co., Ltd. (Shanghai, China). ..

    Blocking Assay:

    Article Title: Mitochondria-Targeted Au@Carbon Dot Nanoprobes for SERS Analysis of Drug-Induced Mitophagy
    Article Snippet: Hydrogen tetrachloroaurate(III) trihydrate (HAuCl4·3H2O) and triphenylphosphine were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). .. The mitochondrial green fluorescent probe JC-1, Enhanced Mitochondrial Membrane Potential Assay Kit (JC-1), SDS-PAGE Gel Preparation Kit, BeyoColor Prestained Protein Marker, RIPA Lysis Buffer (Strong), PMSF, BCA Protein Concentration Assay Kit (Enhanced), HRP-conjugated Goat Anti-Mouse IgG (H + L), HRP-conjugated Goat Anti-Rabbit IgG (H + L), QuickBlock Western Primary Antibody Dilution Buffer, QuickBlock Western Secondary Antibody Dilution Buffer, Western Wash Buffer (10X), QuickBlock Western Blocking Buffer, and Ultrasensitive ECL Chemiluminescence Kit were acquired from Beyotime Biotechnology Co., Ltd. (Shanghai, China). ..



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    Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). <t>(F)</t> <t>SDS-PAGE</t> analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.
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    Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). <t>(F)</t> <t>SDS-PAGE</t> analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.
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    Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). <t>(F)</t> <t>SDS-PAGE</t> analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.
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    Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). <t>(F)</t> <t>SDS-PAGE</t> analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.
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    Image Search Results


    Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). (F) SDS-PAGE analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.

    Journal: Bioactive Materials

    Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis

    doi: 10.1016/j.bioactmat.2025.11.047

    Figure Lengend Snippet: Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). (F) SDS-PAGE analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.

    Article Snippet: Hoechst 33342, DAPI solution, Lyso-Tracker Green, Cell Counting Kit-8 (CCK-8), Calcein-AM/PI Live/Dead cell double staining kit, membrane and cytosol protein extraction kit, BCA kit, and SDS-PAGE gel preparation kit were procured from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Techniques: Zeta Potential Analyzer, Fluorescence, Microscopy, SDS Page, Membrane, Staining, Labeling, Conjugation Assay, In Vitro